Industrial Biotechnology

Plenary Lecture

Novel Enzymes and Microorganisms

11:00-11:10Chair/IntroductionDr. Roland Wohlgemuth, Chairman EFB Section on Applied Biocatalysis, Sigma-Aldrich, Switzerland

11:10-11:35Stereospecific and regiospecific biotransformation Microbial and enzymatic processes for the production of useful chemicalsProf. Dr. Sakayu Shimizu, Proffesor, Division of Applied Life Sciences, Kyoto University, Japan

11:35-12:00Unusual enzymes in polyketide antibiotic biosynthesisProf. Peter Leadlay, Department of Biochemistry, University of Cambridge, UK

12:00-12:25Biocatalysis: Synthesis of Chiral Pharmaceutical Intermediates Dr. Ramesh Patel, Director, Enzyme Technology, Process Research & Development, Bristol-Myers Squibb, USA

12:25-12:50Discovery of Novel Industrial Enzymes and MicroorganismsDr. Wenping Wu, Director, Microbial Discovery Unit, Novozymes, Denmark

12:50-13:00Discussion & Conclusions 

Biocatalyst Function and Optimization

15:00-15:10Chair/IntroductionProf. Jennifer Littlechild, Professor of Biological Chemistry and Director of the Henry Wellcome Centre for Biocatalysis at University of Exeter, UK

15:10-15:35Cyclase enzymesProf. Seiichi P.T. Matsuda, Department of Chemistry, Rice University, USA

15:35-16:00Directed Evolution of Enzymes for Synthetic ApplicationsProf. Nicholas Turner, Professor of Chemical Biology & Director of the Centre of Excellence in Biocatalysis (CoEBio3), Manchester Interdisciplinary Biocentre, UK

16:00-16:25An engineered mutant, L307V of phenylalanine dehydrogenase from Bacillus sphaericus: high activity and stability in organic-aqueous solvent mixtures and utility for synthesis of non-natural L-amino acidsProf. Paul Engel, Professor of Biochemistry, Conway Institute, University College Dublin, Ireland

16:25-16:50Enabling bio-based processes: From biocatalyst discovery to novel “designer” microorganismsDr. Jürgen Eck, Member of the Managing Board and Head of Research & Development (CTO), B.R.A.I.N AG, Germany

16:50-17:00Discussion & Conclusions

Plenary Lecture

Plenary Lecture

Biocatalytic Process Design

11:00-11:10Chair / IntroductionDr. Marcel Wubbolts, DSM Delft, The Netherlands

11:10-11:35Solving key issues in scale-up of bioprocesses.Prof. Dr. Andreas Liese, Head of Institute of Technical Biocatalysis, Hamburg University of Technology (TUHH), Germany

11:35-12:00Challenges for Commodity Chemical Production using Enzyme CatalystsDr. Robert DiCosimo, Senior Research Fellow, DuPont Central Research & Development, USA

12:00-12:25Cross-linked enzyme aggregates (CLEAs): effective catalysts for biotransformationsProf. Roger Sheldon, Delft University of Technology / CLEA Technologies BV, The Netherlands

12:25-12:50Accelerating biocatalytic process design: Integrating new tools from biology, chemistry and engineeringDr. Frank Baganz, Department of Biochemical Engineering, University College London, UK

12:50-13:00Discussion & Conclusions 

Innovative Downstream Processing

15:00-15:10Chair/IntroductionDr. Alois Jungbauer, Dept. Biotechnology, University of Natural Resources & Applied Life Sciences, Austria

15:10-15:35The downstream self-assembly bioprocessing of virus-like vaccinesDr Linda Lua, Manager of the SRC Protein Expression Facility, Institute for Molecular Bioscience at the University of Queensland, Australia

15:35-16:00Monolith chromatography: an efficient method for the separation and purification of biomacromolecules and nanoparticlesDr. Aleš Podgornik, R&D Manager, BIA Separations d.o.o., Slovenia

16:00-16:25Production of recombinant proteins in E.coli: Engineering of batch and continuous refolding processesDr. Alois Jungbauer, Dept. Biotechnology, University of Natural Resources & Applied Life Sciences, Austria

16:25-16:50Integrated process for protein modification using smart magnetic handlesDr. Timothy Hobley, Associate Professor, Technical University Denmark

16:50-17:00Discussion & Conclusions

Plenary Lecture

Plenary Lecture

Metabolic Engineering & Applied Systems Biology

11:00-11:10Chair/IntroductionProf. Uwe Sauer, Institute of Molecular Systems Biology, ETH Zurich, Switzerland

11:10-11:35Bakers’ yeast for biofuel: Engineering Saccharomyces cerevisiae for pentose fermentationProf. Dr. Jack Pronk, Scientific Director, Kluyver Centre for Genomics of Industrial Fermentation; Delft University of Technology, The Netherlands

11:35-12:00Using Transcriptional Engineering to Elicit New Multigenic Phenotypes in MicrobesProf.Dr.Gregory Stephanopoulos, Professor, Department of Chemical Engineering, Massachusetts Institute of Technology, USA

12:00-12:25Rational Design of Efficient MicroorganismsProf. Friedrich Srienc, Biotechnology Institute, Department of Chemical Engineering and Materials Science, University of Minnesota, USA

12:25-12:50Metabolic Engineering of Saccharomyces cerevisiae Microbial Cell Factories for Succinic Acid ProductionMr. Jose Otero, Center for Microbial Biotechnology, BioCentrum-Technical University of Denmark

11:35-12:00Discussion & Conclusions

Fermentation Science & Engineering

15:00-15:10Chair/IntroductionProf. Dr.-Ing. Dirk Weuster-Botz, Institute of Biochemical Engineering, Technical University of Munich, Germany

15:10-15:35A framework for the rapid optimisation and scale-up of protein expression combining microscale experiments and statistical experimental design: Application to protein-based vaccine productionProf. Gary Lye, Professor of Biochemical Engineering, University College London, UK

15:35-16:00Fed-batch operation and pH control in shake flask and micro titre plate cultures by slow release systemsProf. Dr. Jochen Buechs, Head of Department, Biochemical Engineering, RWTH Aachen University, Germany

16:00-16:25Sensor arrays for fermentation monitoringProf. Dr. J.G.E. Gardeniers, Professor in Mesoscale Chemical Systems, MESA+ Institute for Nanotechnology, The Netherlands

16:25-16:50High-throughput process development for recombinant proteins - possibilities, tools, and challengesProf. Peter Neubauer, Professor for Bioprocess Engineering, University of Oulu, Finland

16:50-17:00Discussion & Conclusions

Plenary Lecture